Journal: Scientific Reports

Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV

doi: 10.1038/s41598-023-35328-9

Figure Lengend Snippet: Generation and characterization of Gn protein and rAd5-Gn. ( a ) Schematic diagram of the SFTSV GnGc, Gn∆TM, and Gn∆STEM. ( b ) Predicted structure of Gn∆TM and Gn∆STEM by SWISS-MODEL . A black arrow indicates a different site for GnΔTM and GnΔSTEM. ( c ) SDS-PAGE of purified GnΔTM and GnΔSTEM. ( d ) ELISA binding curves of MAb4-5 to Gn∆TM, Gn∆STEM, and Gn coated at equimolar concentrations. The average ± SD from at least two independent experiments performed is shown. ( e ) Examining the incorporation of GnΔTM into the adenovirus type 5 by western blot analysis. Un.

Article Snippet: Recombinant adenoviruses encoding the SFTSV Gn protein were purchased from SIRION Biotech (Munich, Germany).

Techniques: SDS Page, Purification, Enzyme-linked Immunosorbent Assay, Binding Assay, Western Blot

Journal: Tropical Medicine and Infectious Disease

Article Title: Crimean–Congo Hemorrhagic Fever Virus Survey in Humans, Ticks, and Livestock in Agnam (Northeastern Senegal) from February 2021 to March 2022

doi: 10.3390/tropicalmed7100324

Figure Lengend Snippet: Risk factors for CCHFV in human populations.

Article Snippet: For this, CCHFV recombinant glycoprotein Gn (Sinobiological) was diluted at 50 ng in PBS1x (Sigma-Aldrich) and added in plates, then these plates were incubated overnight at 4 °C for coating.

Techniques:

Journal: Tropical Medicine and Infectious Disease

Article Title: Crimean–Congo Hemorrhagic Fever Virus Survey in Humans, Ticks, and Livestock in Agnam (Northeastern Senegal) from February 2021 to March 2022

doi: 10.3390/tropicalmed7100324

Figure Lengend Snippet: Temporal distribution of anti-CCHFV antibody in livestock sheep. Legend: * on month indicates the rainy seasons.

Article Snippet: For this, CCHFV recombinant glycoprotein Gn (Sinobiological) was diluted at 50 ng in PBS1x (Sigma-Aldrich) and added in plates, then these plates were incubated overnight at 4 °C for coating.

Techniques:

Journal: Tropical Medicine and Infectious Disease

Article Title: Crimean–Congo Hemorrhagic Fever Virus Survey in Humans, Ticks, and Livestock in Agnam (Northeastern Senegal) from February 2021 to March 2022

doi: 10.3390/tropicalmed7100324

Figure Lengend Snippet: Risk factors for CCHFV in livestock sheep.

Article Snippet: For this, CCHFV recombinant glycoprotein Gn (Sinobiological) was diluted at 50 ng in PBS1x (Sigma-Aldrich) and added in plates, then these plates were incubated overnight at 4 °C for coating.

Techniques: